Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc PBS, 10X, PH7.4, 1 L BOTTLE
10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 1 Liter bottle.
FUJIFILM BIOSCIENCES INC MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 25g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 25g
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 240 Grams of Powder, Makes 50 Liters of Solution
Murashige & Skoog (MS) Medium with MES Buffer is a powdered medium formulated for plant tissue culture applications. The 240g powder makes 50 liters of nutrient solution, enriched with MES buffer to stabilize the pH during plant cell culture. The inclusion of MES ensures better pH stability during long incubation periods, especially in environments where pH fluctuations can affect cell growth. This medium is ideal for research in plant biotechnology, as it supports both the growth and differentiation of plant cells, tissues, and organs. It is commonly used for in vitro studies, propagation, and transformation of various plant species.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | 7365-45-9 | MFCD00006158 | 20ML
Medchemexpress LLC L-Adenosine | 3080-29-3 | 99.8% | 1 ML
L-Adenosine is a metabolically stable enantiomeric analog and a potential probe. It exhibits weakly inhibitory adenosine deaminase (ADA) activity with a Ki value of 385 μM. This compound can be utilized for research into adenosine uptake and accumulation.
- Metabolically stable enantiomeric analog.
- Potential probe for research.
- Weakly inhibits adenosine deaminase activity.
- Can be used for adenosine uptake research.
- Can be used for adenosine accumulation research.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra, for molecular biology, >=99.5% (T) | 5625-37-6 | MFCD00006159 | 50G
PIPES BioXtra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 50G
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 8.5) - 250ml
Bicine Buffer (0 5 M pH 8 5) - 250ml (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences CAPS >=99% | 1135-40-6 | MFCD00003837 | 6X100G
CAPS >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 6X100G