Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hampton Research HR2-817/1.0 M ADA pH 6.5 - 100 ml
HR2-817/1.0 M ADA pH 6.5 - 100 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences CAPS BioXtra, >=99% | 1135-40-6 | MFCD00003837 | 100G
CAPS BioXtra, >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 100G
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100 g
PIPES | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 100 g
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
Research Products International Corp DPBS 1X Solution, 500 Milliliters
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Ready to use sterile 1X solution.
Ingredients:
Potassium Chloride: 2.7 mM
Phosphate Buffer: 11.9 mM
pH: 7.4
Does not contain Calcium or Magnesium
Teknova MES Dilution Buffer, pH 6.5. 1000mL, Sterile 8 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Sigma Aldrich Fine Chemicals Biosciences TAPS BioXtra 99.5 titratio1KG
TAPS BioXtra 99.5 titratio1KG
Bioworld BES Buffer, 0.5M, pH 7.5, 500 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Research Products International Corp DIPSO 100 G
Substance Name: 3-(N,N-Bis(2-Hydroxyethyl)amino)-2-hydroxypropane sulfonic acid
CAS Number: 68399-80-4
Molecular Formula: C7H17NO6S
Molecular Weight: 243.3
Chemical Purity: >98%
Soluble in:?Water
Store at:?Room Temperature
Crescent Chemical Co Inc HYDROXYETHYL)PIPERAZINE-N'-2-E
Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid -Na-salt analytical grade pKa 20 = 7.55. Buffering substance for biochemistry and molecular biology. Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 25249.04 CAS 75277-39-3
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt | 71119-22-7 | MFCD00064350 | 250g
MOPS sodium salt | 99.5% | MW: 231.25 | 71119-22-7 | MFCD00064350 | 250g
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660092.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 1 Kg
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a specialized biochemical reagent used for preparing buffer solutions that maintain a stable pH in laboratory experiments. This 1kg formulation is ideal for high-volume applications, including protein chemistry, enzyme assays, and microbiological studies. The sesquisodium salt form of PIPES offers enhanced buffering capacity and is commonly used in research that requires precise pH control, such as in cell culture, protein purification, and electrophoresis. The buffer solution maintains a stable pH around 7.0, which is essential for maintaining enzyme activity and cellular functions during experiments.
Bioworld Bicine, 500 g
A zwitterionic amino acid buffer agent used for low temperature work Technical Information:Synonym: N,N-Bis(2-hydroxyethyl)glycine Titration: > 98.0% (0.1N NaOH)Water content: < 2.0%Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.Appearance: PowderBoiling Point : 388.55 °C at 760 mmHgDensity : 1.05 g/cm3 at 20 °C (lit.)HS Code : 29225000Melting Point: 190 °C (dec.)(lit.)PH Value: 4.3-5.3 (1%)RTECS Number: MB9700000
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661641.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid) is a biochemical reagent used for pH buffering in biochemical applications. This 1 kg volume is ideal for laboratories requiring large quantities of MES buffer for a variety of experiments. MES is commonly used in biochemical research, especially in enzyme assays, cell culture, and protein purification. It maintains a stable pH range, crucial for experiments that need precise and consistent conditions. The MES buffer ensures minimal interference in reactions, making it ideal for applications such as electrophoresis and chromatography. MES Monohydrate is a reliable and effective reagent used in a wide range of scientific studies, particularly in molecular biology and biochemistry.