Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. N-(2-Acetamido)-2-aminoethanesulfonic acid | 7365-82-4 | MFCD00008030 | 250G
N-(2-Acetamido)-2-aminoethanesulfonic acid, 7365-82-4, MFCD00008030, 250G
Chem-Impex International, Inc. N-(2-Acetamido)-2-aminoethanesulfonic acid | 7365-82-4 | MFCD00008030 | 1KG
N-(2-Acetamido)-2-aminoethanesulfonic acid, 7365-82-4, MFCD00008030, 1KG
Bioworld PBS/Fish Gelatin (10X), 200 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)
Bioworld CAPS, Transfer Buffer (5X), 1 L
Product DescriptionCAPS-based transfer buffer at 5X contains a concentration between 90-110 mM CAPS and 10% methanol, with a pH value between 10.8-11.2 at 25C°. Buffer may be preferable for transfers of high molecular weight proteins (such as >150 kD) and in cases where the glycine component in buffer may interfere with downstream protein sequencing applications. Protease (P/F): None
Bioworld PBS/Fish Gelatin (10X), 500 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 1 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Research Products International Corp Tris Glycine Buffer 10x Powdr
Dry homogeneous blend of molecular biology grade TRIS base and Glycine. Add 174.4g to make 1L of 10x Concentrate.
Hampton Research HR2-727/1.0 M TRIS hydrochloride pH 8.5 - 100 ml
HR2-727/1.0 M TRIS hydrochloride pH 8.5 - 100 ml. Please note items from this supplier are non-returnable.
Hampton Research HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml
HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Hampton Research HR2-781/1.0 M BIS-TRIS pH 5.5 - 100 ml
HR2-781/1.0 M BIS-TRIS pH 5.5 - 100 ml. Please note items from this supplier are non-returnable.
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 5kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .